Next-generation sequencing (NGS) is a high-throughput methodology that enables rapid sequencing of the base pairs in DNA or RNA samples to study genomic variation associated with cancer or other diseases. NGS allows massive parallel sequencing of hundreds and thousands of genes or whole genome in a short period of time. Supporting a broad range of applications, including gene expression profiling, detection of epigenetic changes and molecular analysis.
More recently, NGS has been adopted in Clinical Oncology to identify biomarkers for early diagnosis, prognosis and therapeutic decision. In addition, NGS is used to identify novel and rare cancer mutations, detect familial cancer mutation carriers and provide molecular rationale for appropriate targeted and personalized precision treatment of cancer.